The Wales international may have therefore played his final game for the Gunners, having agreed to join Juventus when he becomes a free agent in the summer.
Ramsey went off with a hamstring injury during the second leg of Arsenal's Europa League quarter-final win over Napoli but it was hoped he may recover before the end of the season.
However, he did not train on Wednesday ahead of Thursday's semi-final first leg against Valencia and will not be fit in time for a possible final - or Arsenal's two remaining Premier League fixtures.
Ramsey is Arsenal's longest-serving player, having joined from Cardiff back in 2008.
He has made over 350 appearances for the club, twice scoring the winner in FA Cup finals.
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